Development of validated UHPLC–PDA with ESI–MS-MS method for concurrent estimation of magnoflorine, berbamine, columbamine, jatrorrhizine, palmatine and berberine in Berberis aristata

Abstract A validated UHPLC-PDA with an ESI-MS/MS method has been developed for simultaneous estimation of six bioactive alkaloids (magnoflorine, berbamine, columbamine, jatrorrhizine, palmatine and berberine) in the different extracts roots Berberis aristata DC (Family:Berberdiaceae). It is important medicinal herb native to Northern Himalaya commonly known as ‘daruharidra’, ‘daruhaldi’, ‘Indian barberry’ or ‘tree turmeric’. An insight into research literature uncovered reports on isoquinoline like magnoflorine, palmatine, berberine major bioactives B. roots, possessing pharmacological therapeutic effects. In present study, these aforementioned were separated Phenomenex Luna®, 5 µm-C8 analytical column. The HPLC-MS analysis was performed at a flow rate 0.90 mL min −1 . Each alkaloid that resolved characterized by precursor ions fragment electrospray ionization (ESI) source both positive negative using scan mode. limit detections (LODs) 0.087, 0.727, 0.035, 0.124, 0.782 0.794 μg berberine, respectively. proposed fully according international (ICH) guidelines found be selective, sensitive highly accurate concomitant symbolic bio-markers roots.